Figure 2.
Figure 2. Some ECL1 mutants exhibited decreased burst duration. (A) Representative single-channel current traces and their all-points histograms for WT-, D110R-, D112R-, K114D-, E115R-, E116R-, and R117A-CFTR from inside-out membrane patches excised from Xenopus oocytes, with symmetrical 150 mM Cl− solution in the presence of 1 mM MgATP and 50 U/ml PKA. All traces were recorded at VM = −100 mV. c, closed state; f, full open state; s1 and s2, subconductance states 1 and 2. The solid lines in the histograms are fit results to a Gaussian function. (B and C) Single-channel amplitudes of the full open state (B) and mean burst durations (C) of WT-, D110R-, D112R-, K114D-, E115R-, E116R-, and R117A-CFTR. (D) Apparent open probability of WT-, D110R-, E116R-, and R117A-CFTR. *, P < 0.05 compared with WT-CFTR; #, P < 0.001 compared with WT-CFTR. n = 4–7 for all variants. Mean ± SEM is shown. This analysis reflected only openings to the f state.

Some ECL1 mutants exhibited decreased burst duration. (A) Representative single-channel current traces and their all-points histograms for WT-, D110R-, D112R-, K114D-, E115R-, E116R-, and R117A-CFTR from inside-out membrane patches excised from Xenopus oocytes, with symmetrical 150 mM Cl− solution in the presence of 1 mM MgATP and 50 U/ml PKA. All traces were recorded at VM = −100 mV. c, closed state; f, full open state; s1 and s2, subconductance states 1 and 2. The solid lines in the histograms are fit results to a Gaussian function. (B and C) Single-channel amplitudes of the full open state (B) and mean burst durations (C) of WT-, D110R-, D112R-, K114D-, E115R-, E116R-, and R117A-CFTR. (D) Apparent open probability of WT-, D110R-, E116R-, and R117A-CFTR. *, P < 0.05 compared with WT-CFTR; #, P < 0.001 compared with WT-CFTR. n = 4–7 for all variants. Mean ± SEM is shown. This analysis reflected only openings to the f state.

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