Cross-linking alters ENaC open probability. (A–D) Representative single-channel current traces (cell-attached configuration) obtained at −100 mV (110 mM LiCl in bath and pipette) in oocytes expressing αK477CβV85CγENaC (A and B) or αK477CβV85CγR135A, R137A, R138A, R178W, R179A, R180A, R181AENaC (C and D) without (top traces) or with (bottom traces) MTS-14-O4-MTS pretreatment. (B) Open probability determined from patches described in A (n = 5–8; *, P < 0.045). (D) Open probability determined from patches described in C (n = 5; *, P < 0.025). (E) Representative single-channel current traces (cell-attached configuration) obtained at 60 mV (110 mM LiCl in bath and 1 mM LiCl in pipette) in oocytes expressing αK477CβV85CγENaC without (top trace) or with (bottom trace) MTS-2-MTS pretreatment. (F) Open probability determined from patches described in E (n = 4–7; *, P < 0.015). Mean ± SEM is shown.
