Figure 5.
Figure 5. Spontaneous disulfide bond reduces ENaC current. (A) Representative current trace (voltage clamped to −60 mV) in a Xenopus oocyte expressing αL120CβγE455CENaC. Amiloride-sensitive ENaC current was measured before and after treatment with 30 mM DTT (white bar). ENaC current was determined by block with 10 µM amiloride (Amil, black bars). (B) Percent change in ENaC current induced by DTT in oocytes expressing αβγENaC (subunits are wild type or the indicated mutants). Mean ± SEM is shown (n = 6–7; *, P < 0.002).

Spontaneous disulfide bond reduces ENaC current. (A) Representative current trace (voltage clamped to −60 mV) in a Xenopus oocyte expressing αL120CβγE455CENaC. Amiloride-sensitive ENaC current was measured before and after treatment with 30 mM DTT (white bar). ENaC current was determined by block with 10 µM amiloride (Amil, black bars). (B) Percent change in ENaC current induced by DTT in oocytes expressing αβγENaC (subunits are wild type or the indicated mutants). Mean ± SEM is shown (n = 6–7; *, P < 0.002).

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