Figure 3.
![Figure 3. Cross-linking alters ENaC current. (A, B, D, and E) Representative current traces (voltage clamped to −60 mV) in oocytes expressing wild-type αβγENaC (A), αK477CβV85CγENaC (B), αβE446CγV87CENaC (D), and αL120CβγE455CENaC (E). Records show ENaC current before (blue lines) and after treatment with MTS-14-O4-MTS (in the presence of amiloride [Amil]). (C and F) Percent change in ENaC current induced by MTS-14-O4-MTS in oocytes expressing αβγENaC (subunits are wild type or the indicated mutants). Mean ± SEM is shown (n = 6–12; *, P < 0.001).](https://cdn.rupress.org/rup/content_public/journal/jgp/144/4/10.1085_jgp.201411208/5/jgp_201411208_fig3.jpeg?Expires=1773469154&Signature=GT9SVPnbC4D7qcAtybqXN~4OyVvGqsiFxpcDLJcjprr8Pko-pW1O3FEKKXgX0vXngknPyn8oXWPvEeegMqBrDO9KPqOZexVB8lFMmBbJLxgpU069~EwAhBFMhsmr6AGDwvmy9xx3Jt6~oYiUmMI2XytA41UXCE6K0b9y~QnwxEQ9TLmPhBygSKs3NFXWpZoBzpcPnbvwSob48TT7xpM~IdnFlK7ng-wuGuI8WwqKyS5~u6HRp-6z0Ipe409J40iVEr6Bo1IcQsF~uVC7agDQ5HN6Bo4nFKfhDrmsrxZloDdVZ9L70zMy~OxXUNsbWds8obFNx-uNzJOnqxojFSstjw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cross-linking alters ENaC current. (A, B, D, and E) Representative current traces (voltage clamped to −60 mV) in oocytes expressing wild-type αβγENaC (A), αK477CβV85CγENaC (B), αβE446CγV87CENaC (D), and αL120CβγE455CENaC (E). Records show ENaC current before (blue lines) and after treatment with MTS-14-O4-MTS (in the presence of amiloride [Amil]). (C and F) Percent change in ENaC current induced by MTS-14-O4-MTS in oocytes expressing αβγENaC (subunits are wild type or the indicated mutants). Mean ± SEM is shown (n = 6–12; *, P < 0.001).
