Figure 2. Cross-linking of intersubunit... | Rockefeller University Press

Figure 2.

$Figure 2. Cross-linking of intersubunit residues. (A–C) Representative immunoblots of βENaC (A and B) and γENaC (C) after isolation of the biotinylated cell surface fraction from HEK 293 cells expressing the indicated ENaC subunits. Cells were treated with DMSO (control), MTS-14-O4-MTS, MTS-2-MTS, or DTT, as indicated. Molecular mass is indicated in kilodaltons. Models illustrate the three ENaC subunits (viewed from the top) and locations of cross-linked residues.$

Cross-linking of intersubunit residues. (A–C) Representative immunoblots of βENaC (A and B) and γENaC (C) after isolation of the biotinylated cell surface fraction from HEK 293 cells expressing the indicated ENaC subunits. Cells were treated with DMSO (control), MTS-14-O4-MTS, MTS-2-MTS, or DTT, as indicated. Molecular mass is indicated in kilodaltons. Models illustrate the three ENaC subunits (viewed from the top) and locations of cross-linked residues.

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