Voltage-dependent anion transport of CLC^Fs. (A and B) Anion efflux by CLC-pst with K⁺/Na⁺ conditions indicated. Efflux time courses are normalized to the values after disrupting the liposomes with detergent. (C and D) Cl⁻ transport via CLC-pst monitored by light scattering, with either Vln (C) or gA (D) added to initiate transport. (E and F) Voltage dependence of anion transport for CLC-pst and CLC-eca, respectively. Initial rates for F⁻ and Cl⁻ were normalized to the initial rate for F⁻ transport at −151 mV. Voltage values are calculated from the K⁺ Nernst potential set by varying external K⁺ with fixed internal K⁺.