Generalized interactions between binary elements. (A) Kinetic scheme represented by a pair of binary elements i and j, where the transition rates are labeled with the rate constants α and β for each element, and all of the possible generalized interaction factors as defined in Eqs. 7–12. (B) Energy landscape correlate of the kinetic scheme in A, where transitions are only allowed along the edges between states. The i0j0 state is set as the zero energy reference state, and the relative energies of the remaining three states and the transition state intermediates between states are labeled with the system parameter that defines them as described in the Materials and methods. Bold broken lines indicate the energy landscape in the absence of any interaction between the elements, the deviation from which is described by the respective allosteric (equilibrium) and catalytic interaction energies.
