Figure 8. The interactions between... | Rockefeller University Press

Figure 8.

$Figure 8. The interactions between AKAP150 and TRPV4 channels are dynamic. (A) FRAP of AKAP150-EGFP in a small region of interest (diameter = 1 µm) of a representative tsA-201 cell. The gray rectangle shows the bleaching period with high-intensity 474 nm light. The broken line marks the mean AKAP150-EGFP fluorescence intensity before bleaching. fm and fi are mobile and immobile fractions, respectively. (B) Bar plots of the mobile and immobile fractions. Data are means ± SEM (error bars; n = 5). (C) Confocal images of the top and center of a cell expressing TRPV4-mCherry and AKAP150-EGFP. Bars, 2 µm. (D) RICS auto (TRPV4 and AKAP150) and cross-correlation (ccRICS) signal. (E) RICS auto (CIBN and AKAP79) and cross-correlation (ccRICS) signal. Bars, 2 µm.$

The interactions between AKAP150 and TRPV4 channels are dynamic. (A) FRAP of AKAP150-EGFP in a small region of interest (diameter = 1 µm) of a representative tsA-201 cell. The gray rectangle shows the bleaching period with high-intensity 474 nm light. The broken line marks the mean AKAP150-EGFP fluorescence intensity before bleaching. f_m and f_i are mobile and immobile fractions, respectively. (B) Bar plots of the mobile and immobile fractions. Data are means ± SEM (error bars; n = 5). (C) Confocal images of the top and center of a cell expressing TRPV4-mCherry and AKAP150-EGFP. Bars, 2 µm. (D) RICS auto (TRPV4 and AKAP150) and cross-correlation (ccRICS) signal. (E) RICS auto (CIBN and AKAP79) and cross-correlation (ccRICS) signal. Bars, 2 µm.