Super-resolution imaging reveals clustering of TRPV4 channels in arterial myocytes. (A) Confocal image of a representative arterial myocyte labeled with TRPV4-specific antibody and the corresponding secondary antibody. (B) TIRF image (i) and super-resolution localization maps (ii and iii) of an exemplar arterial myocyte labeled with a TRPV4-specific antibody. iii shows an expanded view of the region of the cell in ii marked by the rectangle. The insets in iii highlight TRPV4 channel clusters in regions a, b, and c of the myocyte. Inset bar, 200 nm. (C) Histogram of the area of TRPV4 channel clusters in super-resolution localization maps.