CG-MALS protocols. Samples are mixed just before entering the flow cell where light scattering is measured. The composition of the sample in the flow chamber over time is controlled by the following protocols: (A) Self+hetero-association protocol is used to determine the presence of homo-oligomers and hetero-oligomers. The concentration of SKp increases with steps until its maximum concentration is reached. A broad range of molar ratios of SKp:CaM are achieved by changing the composition of the mixture at each step with decreasing SKp, but increasing CaM. When SKp is zero and CaM is at its maximum, homo-association of CaM is evaluated by decreasing CaM concentration at each step. (B) Dual crossover protocol is used to evaluate hysteresis of binding. SKp concentration is shown in black and CaM concentration is shown in orange.