Functional dissociation constants of PI(4,5)P₂ binding to TRPC3/6/7 channels. (A) Comparison of FR or E_EFF in the resting condition between cells expressing TRPC6 channel and CFP_mse-PHd and YFP_mse-PHd (control), and those overexpressing PIP5K (+PIP5K). The FR of cells overexpressing PIP5K increased on average by ∼1.2-fold compared with control cells. *, P < 0.05; unpaired t test. (B) Steady-state plots for estimating the functional K_d of PI(4,5)P₂ binding to TRPC3/6/7 channels. Horizontal axis indicates the estimated PI(4,5)P₂ concentration based on the conversion from FR to PI(4,5)P₂, according to Eq. 5.