Figure 4.
![Figure 4. Overexpression of dominant-negative CaM1,2,3,4 or CaM2,3,4 has no effect on Ano1 current amplitude or kinetics but suppresses SK2 currents. (A and D) Current traces from cells expressing mAno1(abc) (A) or SK2 (D) with or without CaM1,2,3,4 or CaM2,3,4. (B, C, E, and F) Steady-state current-voltage relationships for Ano1(abc) (B and C) or SK2 (E and F) coexpressed with CaM1,2,3,4 (B and E) or CaM2,3,4 (C and F; n = 5–10). To test whether the effect of CaM might vary with [Ca2+], experiments with CaM1,2,3,4 used 20 µM free Ca2+ and experiments with CaM2,3,4, used 1 µM Ca2+. Error bars are ±SEM.](https://cdn.rupress.org/rup/content_public/journal/jgp/143/2/10.1085_jgp.201311047/4/jgp_201311047r_fig4.jpeg?Expires=1767780919&Signature=pkdSc4ZUz0LVIVKI4I9CGk2mQRQylhWExH06FGI8DL6AFflOQLqAiga5dRe395FXnMmC0~KvfB4uxEFnOT9eyhCsSvONX6uxu75aSu2G2AcouO2WxlExSNRGLB82orRd-4zZUhiYGXtITMjIh5Hm~q0iVova7U6NavM9QlCwmUqt36OYza4d-r8aiv9DJkMIXuK2BzYuoPWoZm-bJqGJl4pmG6pHh9ZtJiOBZYOMfnA9J~F3TOwTnsnGHArHB3mlkfD6l03lGYBKcIp93d~d7lUrVHS9l77m41UztsCAuYpA6SnQ9KL3UyjhfzfwkCINcki926URtIlGKtOJBzGT-Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Overexpression of dominant-negative CaM1,2,3,4 or CaM2,3,4 has no effect on Ano1 current amplitude or kinetics but suppresses SK2 currents. (A and D) Current traces from cells expressing mAno1(abc) (A) or SK2 (D) with or without CaM1,2,3,4 or CaM2,3,4. (B, C, E, and F) Steady-state current-voltage relationships for Ano1(abc) (B and C) or SK2 (E and F) coexpressed with CaM1,2,3,4 (B and E) or CaM2,3,4 (C and F; n = 5–10). To test whether the effect of CaM might vary with [Ca2+], experiments with CaM1,2,3,4 used 20 µM free Ca2+ and experiments with CaM2,3,4, used 1 µM Ca2+. Error bars are ±SEM.
