Figure 2. AKAP15 and AKAP79 compete for binding to the DCT of CaV1.2 channels. (A) Representative IBa through CaV1.2Δ1800 and CaV1.2Δ1800 + DCT channels coexpressed with cDNA ratios of 0.003:1 WT AKAP15 and/or 0.01:1 AKAP79 in the presence of 5 µM forskolin (Fsk) elicited by a test pulse to 10 mV from a holding potential of −80 mV. (B) Mean current-voltage relationships for CaV1.2Δ1800 and CaV1.2Δ1800 + DCT channels coexpressed with 0.003:1 WT AKAP15 and/or 0.01:1 AKAP79 and 5 µM forskolin. Error bars are SEM. (C) Coupling efficiency (nA/pC) for CaV1.2Δ1800 and CaV1.2Δ1800 + DCT channels coexpressed with 0.003:1 WT AKAP15 and/or 0.01:1 AKAP79 and 5 µM forskolin. Dashed line indicates mean coupling ratio for unstimulated CaV1.2Δ1800 + DCT. n values and mean ± SEM are indicated. **, P < 0.01 versus control. Significance was determined by ANOVA.
Figure 2.

AKAP15 and AKAP79 compete for binding to the DCT of CaV1.2 channels. (A) Representative IBa through CaV1.2Δ1800 and CaV1.2Δ1800 + DCT channels coexpressed with cDNA ratios of 0.003:1 WT AKAP15 and/or 0.01:1 AKAP79 in the presence of 5 µM forskolin (Fsk) elicited by a test pulse to 10 mV from a holding potential of −80 mV. (B) Mean current-voltage relationships for CaV1.2Δ1800 and CaV1.2Δ1800 + DCT channels coexpressed with 0.003:1 WT AKAP15 and/or 0.01:1 AKAP79 and 5 µM forskolin. Error bars are SEM. (C) Coupling efficiency (nA/pC) for CaV1.2Δ1800 and CaV1.2Δ1800 + DCT channels coexpressed with 0.003:1 WT AKAP15 and/or 0.01:1 AKAP79 and 5 µM forskolin. Dashed line indicates mean coupling ratio for unstimulated CaV1.2Δ1800 + DCT. n values and mean ± SEM are indicated. **, P < 0.01 versus control. Significance was determined by ANOVA.

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