Additional characterization of rejuvenation interventions and aging mutant mice. (A) Lineage distribution at 4 mo after transplantation in both blood and BM for the exercise transplantation experiment shown in Fig. 9 E (left) and calorie restriction transplantation experiment shown in Fig. 9 J (right). (B–G) Analysis of 24-mo-old control (Cn) and long-lived Ghrh^−/− mutant mice (Gh) with (B) experimental setup; (C) lineage distribution in the BM of primary animals; and (D) frequency and CD150 levels, (E) regenerative capacity (250 HSCs/recipient), and (F) lineage distribution in primary recipients; and (G) regenerative capacity (250 HSCs/recipient) in secondary transplantation for the indicated HSC populations. Data are means ± SD; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.