Figure 6.
Figure 6. pH-dependent DHA sensitivity. (A) Representative current sweeps in control (black), 2.1 µM DHA (red), 7 µM DHA (orange), 21 µM DHA (yellow), 70 µM DHA (green), and 210 µM DHA (blue) at pH 9.0 and −15 mV (the voltage corresponding to 10% of Gmax in control solution minus 10 mV). Current amplitude is increased >40-fold at 70 µM DHA. (B) Representative G(V) curves. Same cell, color coding, and order as in A. The shifts are −4.9, −15.7, −26.7, −50.0, and −54.6 mV in this example. (C) Dose–response curve for R362Q (gray) and the 3R channel (red) at pH 7.4 (light colored) and at pH 9.0 (dark colored). Error bars indicate SEM (n = 4–15). (D) VSD of the open 3R Shaker structure (top view to the left and side view to the right) with arginines in S4 shown as blue sticks.

pH-dependent DHA sensitivity. (A) Representative current sweeps in control (black), 2.1 µM DHA (red), 7 µM DHA (orange), 21 µM DHA (yellow), 70 µM DHA (green), and 210 µM DHA (blue) at pH 9.0 and −15 mV (the voltage corresponding to 10% of Gmax in control solution minus 10 mV). Current amplitude is increased >40-fold at 70 µM DHA. (B) Representative G(V) curves. Same cell, color coding, and order as in A. The shifts are −4.9, −15.7, −26.7, −50.0, and −54.6 mV in this example. (C) Dose–response curve for R362Q (gray) and the 3R channel (red) at pH 7.4 (light colored) and at pH 9.0 (dark colored). Error bars indicate SEM (n = 4–15). (D) VSD of the open 3R Shaker structure (top view to the left and side view to the right) with arginines in S4 shown as blue sticks.

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