Combinations of arginines make channels more PUFA sensitive. DHA-induced shifts for multicharge channels (70 µM DHA at pH 7.4). Mean ± SEM (n = 9, 12, 10, 15, 11, 8, and 8). Horizontal lines denote shifts of R362Q (black), I360R/R362Q (light blue), and A359R/R362Q (dark blue). DHA-induced shifts compared with R362Q (one-way ANOVA together with Dunnett’s multiple comparison test: *, P < 0.05; **, P < 0.01; ***, P < 0.001).