Sensitivities of hSlo1 Y318S+hβ1 complexes to various fatty acids. (A) Fatty acid structures. DHA (22:6(ω-3)), EPA (20:5(ω-3)), ALA (18:3(ω-3)), AA (20:4(ω-6)), 17OH DHA, and DHA EE. (B) Changes in G-V V_0.5 (left) and fractional changes in Q_app (right) by the fatty acids indicated. The vertical line and the surrounding gray area for each reagent represent the mean ± SEM response from the wild-type hSlo1+hβ1 complex. When corrected for five-way comparisons, the ΔV_0.5 values of hSlo1 Y318S+hβ1 for DHA and those for EPA significantly differ from those of wild-type hSlo1+hβ1 for DHA (P < 10⁻⁷ and 0.02, respectively). (C) Differences in ΔV_0.5 between hSlo1 Y318S+hβ1 and wild-type hSlo1+hβ1 caused by the fatty acids indicated.