DHA fails to alter currents through hSlo1 Y318S+hβ4. (A) Comparison of the activation kinetics of the currents obtained from cells transfected with the hSlo1 Y318S DNA (blue) and the hSlo1 Y318S and hβ4 DNAs together (red). n = 16 and 8 for hSlo1 Y318S and hSlo1 Y318S+hβ4, respectively. The line width indicates SEM. (B) Representative currents measured at two different voltages before (blue) and after (red) the application of DHA. (C) Fractional increases in peak outward currents by DHA. n = 8. (D) G-V curves before (blue) and after (red) the application of DHA. n = 6. The smooth curves are Boltzmann fits to the results. The estimated V_0.5 and Q_app values for the control group are 152.2 ± 0.8 mV and 1.11 ± 0.04, and for the DHA group are 149.5 ± 1.1 mV and 1.00 ± 0.04 (n = 6). DHA was applied at 3 µM. All results were obtained without intracellular Ca²⁺.