Currents through hSlo1 Y318S are largely unaltered by DHA. (A) Representative ionic currents through hSlo1 Y318S at four different voltages (blue, control; red, DHA). (B) Fractional changes in peak outward currents through hSlo1 Y318S by DHA. n = 16. (C) G-V curves before (blue) and after (red) the application of DHA. n = 16. (D) Changes in V_0.5 (left) and Q_app (right) in individual experiments. Neither changes are significant (P = 0.10 and 0.32). (E) Normalized currents through hSlo1 Y318S before (blue) and after (red) the application of DHA at three different voltages from a representative patch. (F) Voltage dependence of time constant of current relaxation before (blue) and after (red) the application of DHA. n = 10–16. (G) Fractional changes in time constant of current relaxation by DHA. n = 10–16. (H) DHA at 3 and 10 µM fails to increase currents through hSlo1 Y318S. Currents were elicited by pulses to 130 mV (G/G_max = ∼0.15), and the peak outward current sizes are plotted. All results were obtained without intracellular Ca²⁺, and, except where indicated, DHA was applied at 3 µM.