G-V parameters of hSlo1+hβ1 complexes bearing human-to-Drosophila mutations in the S5, P/filter, and S6 segments by 3 µM DHA. (A) The results obtained before (blue) and after (red) the addition of DHA are compared. (B) Changes in V_0.5 and fractional changes in Q_app by DHA. When compared with the values in the WT group, only the ΔV_0.5 values for Y318S are statistically distinct (P < 10⁻⁷) after correcting the significance level for 16-way comparisons with the wild-type values. All results were obtained without intracellular Ca²⁺.