Changes in properties of hSlo1+hβ1 complexes bearing human-to-Drosophila mutations in the S5 segment by 3 µM DHA in the absence of intracellular Ca²⁺. For each mutant, sample currents before (blue) and after (red) the application of DHA at two different voltages where the control G/G_max values are ∼0.1 and ∼0.5, fractional increases in peak outward currents at different voltages, and G-V curves before (blue) and after (red) the application of DHA are depicted. The smooth curves are Boltzmann fits to the datasets before and after the application of DHA (see Table 1 for the parameter values).