Currents through human–Drosophila chimeric Slo1 channels. (A) Schematic organizational diagrams of hSlo1 (pink) and dSlo1 (light blue). (B) Currents through four different human–Drosophila chimeric Slo1 channels. For each construct, a schematic organizational diagram, sample currents at three different voltages before (blue) and after (red) the application of 3 µM DHA, and fractional increases in peak outward currents at different voltages, are presented. In the organization diagrams, the pink and light blue regions represent hSlo1 and dSlo1, respectively. In each I_DHA/I_Control graph, the red dashed vertical line indicates the G-V V_0.5 value for the chimeric channel, the black dashed line shows the V_0.5 value of hSlo1, and the dashed gray curve represents the results obtained for hSlo1 (mean ± SEM) for comparison.