Currents through dSlo1 channels do not respond to DHA. (A) Currents through dSlo1 channels in a patch (bottom) elicited by the voltage pulses indicated (top). (B) G-V curves for dSlo1 (red) and hSlo1 (black). The smooth curves represent Boltzmann fits to the datasets. The V_0.5 and Q_app values for dSlo1 are 245.0 ± 1.0 mV and 0.92 ± 0.03 (n = 5), and for hSlo1, the values are 156.2 ± 0.78 mV and 1.27 ± 0.05 (n = 11). (C) Representative currents through dSlo1 elicited by pulses to 140, 170, and 200 mV (top left) and the peak outward current sizes as a function of time (bottom left). DHA was present during the time indicated by the gray horizontal bar. The patch was excised at t = 0. The graph on the right shows the kinetics of current increase in hSlo1 by DHA for comparison (n = 15). (D) Fractional increases in peak outward currents through dSlo1 as a function of voltage (red; n = 17–22, depending on the voltage). The results obtained for hSlo1 are shown using the black curve and the surrounding gray area (mean ± SEM). (E) Fractional increases in peak outward currents through dSlo1 as a function of G/G_max. The results shown in D are transformed using the G-V curves in B. (F) Representative openings of dSlo1 at −120 mV before (top) and after (bottom) the addition of DHA. For each group, 25 1-s segments are superimposed and displayed. (G) Estimated fractional increases in P_o by DHA at different voltages. n = 7 at each voltage. The currents were recorded in the absence of Ca²⁺. DHA was applied at 3 µM.