DHA increases currents through hSlo1 channels in the presence of 300 µM of intracellular Ca²⁺. (A) Representative currents before (blue) and after (red) the application of DHA. No leak current subtraction. (B) Representative I-V curves before (blue) and after (red) the application of DHA. (C) Fractional increases in peak currents at different voltages by DHA. n = 6. (D) G-V curves before (blue) and after (red) the application of DHA. The smooth curves are Boltzmann fits to the results. The V_0.5 and Q_app values for the control condition are −29.3 ± 0.92 mV and 1.48 ± 0.07, and for the results obtained with DHA, the values are −36.4 ± 1.7 mV and 1.25 ± 0.09, respectively. n = 6. The V_0.5 values are statistically different (P = 0.031), but the Q_app values are indistinguishable (P = 0.063). DHA was applied at 3 µM.