DHA increases currents through hSlo1 channels in the absence of intracellular Ca²⁺. (A) Representative hSlo1 currents before (blue) and after (red) the application of DHA to the intracellular side. (B) Fractional increases in peak outward currents at different voltages by DHA. n = 11. (C) Mean G-V curves before (blue) and after (red) the application of DHA. The smooth curves are Boltzmann fits to the datasets. The V_0.5 and Q_app values for the control group are 156.2 ± 0.78 mV and 1.27 ± 0.05, and for the DHA group, they are 145.9 ± 0.86 mV and 1.18 ± 0.04, respectively. n = 11. (D) Changes in V_0.5 and Q_app by DHA in the individual experiments analyzed. The control values are shown in blue, and the values after the addition of DHA are shown in red. The mean values are shown using large diamonds. The V_0.5 values are statistically different before and after DHA application (P < 0.001), but the Q_app values are indistinguishable (P = 0.08). (E) Comparison of the current kinetics at three different voltages before (blue) and after (red) the application of DHA in a representative patch. The currents were scaled to facilitate comparison. (F) Voltage dependence of time constant of current relaxation before (blue) and after (red) the application of DHA. n = 6–9, depending on the voltage. (G) Fractional changes in time constant of current relaxation by DHA at different voltages. n = 6–9, depending on the voltage. DHA was applied at 3 µM.