Figure 8.
Figure 8. Repetitive pulsing decreases QON. (A) From an HP of −90 mV, 80-ms test pulses were delivered at 4 Hz, causing a decrease in the amplitude of ON-sensing currents (ION). Recording was performed at pHEXT 6.5 to minimize charge loss by deprotonation. No changes in kinetics were detected (inset). (B) QON for the mutant R232H and R217Q were calculated by numerical integration of ION. A reduction of 54% in amplitude at the 25th pulse was observed for the R232H mutant, whereas a <10% decrease was observed for R217Q.

Repetitive pulsing decreases QON. (A) From an HP of −90 mV, 80-ms test pulses were delivered at 4 Hz, causing a decrease in the amplitude of ON-sensing currents (ION). Recording was performed at pHEXT 6.5 to minimize charge loss by deprotonation. No changes in kinetics were detected (inset). (B) QON for the mutant R232H and R217Q were calculated by numerical integration of ION. A reduction of 54% in amplitude at the 25th pulse was observed for the R232H mutant, whereas a <10% decrease was observed for R217Q.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal