Figure 6.
Figure 6. Mutant R232H. (A) Sensing currents from the mutant R217Q-R232H-C363S evoked by 400-ms test pulses from −140 to 40 mV with an HP of −90 mV. Sensing current recordings were recorded at pH 7.4. (B) Detail of the ON- and OFF-sensing currents (ION and IOFF, respectively) from the record in A. (C) QON- and QOFF-V curves obtained from numerical integration of the ION (black open squares) and IOFF (red open circles) from the example shown in A. Solid symbols are for the QON (black solid squares)- and QOFF-V (red solid circles) curves for the mutant R217Q from an individual experiment. The fit to a single Boltzmann shows a V1/2 for the QON of −8 mV (error 1 mV) with a slope of 1.33 (error = 0.06). QOFF at 40 mV was 39% of QON,MAX.

Mutant R232H. (A) Sensing currents from the mutant R217Q-R232H-C363S evoked by 400-ms test pulses from −140 to 40 mV with an HP of −90 mV. Sensing current recordings were recorded at pH 7.4. (B) Detail of the ON- and OFF-sensing currents (ION and IOFF, respectively) from the record in A. (C) QON- and QOFF-V curves obtained from numerical integration of the ION (black open squares) and IOFF (red open circles) from the example shown in A. Solid symbols are for the QON (black solid squares)- and QOFF-V (red solid circles) curves for the mutant R217Q from an individual experiment. The fit to a single Boltzmann shows a V1/2 for the QON of −8 mV (error 1 mV) with a slope of 1.33 (error = 0.06). QOFF at 40 mV was 39% of QON,MAX.

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