Figure 4.
Figure 4. Mutant R226H. (A and B) Currents recorded from Xenopus oocytes expressing the mutant R217Q-R226H-C363S were evoked by applying test pulses ranging from −140 to 100 mV from an HP of −90 mV. Decreasing the pHEXT from 7.4 (A, top) to 6.0 (A, bottom) increased the amplitude of ITAIL. (B) Integration of ITAIL yielded QOFF that increased as the pHEXT decreased (see R226H produces a current in the “up” state of the S4 for details). (C) Leak-corrected ISTEP values show that in the active state, the VSD of the mutant R226H conducts protons, as revealed by the change in VREV as a function of pHEXT. Error bars are SD.

Mutant R226H. (A and B) Currents recorded from Xenopus oocytes expressing the mutant R217Q-R226H-C363S were evoked by applying test pulses ranging from −140 to 100 mV from an HP of −90 mV. Decreasing the pHEXT from 7.4 (A, top) to 6.0 (A, bottom) increased the amplitude of ITAIL. (B) Integration of ITAIL yielded QOFF that increased as the pHEXT decreased (see R226H produces a current in the “up” state of the S4 for details). (C) Leak-corrected ISTEP values show that in the active state, the VSD of the mutant R226H conducts protons, as revealed by the change in VREV as a function of pHEXT. Error bars are SD.

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