Figure 3.
Figure 3. Mutant R223H. (A) Current recorded from Xenopus oocytes expressing the mutant R217Q-R223H-C363S. The currents are likely carried by protons as their amplitude increases with the acidification of the external medium. HP was 40 mV, and test pulses ranged from −140 to 50 mV. (B) Current amplitudes at the end of the test pulse were normalized with respect to the end-of-the-step amplitude recorded at −100 mV and pHEXT 7.4. Current displays several-fold increase at pHEXT 6.0, while decreasing at pHEXT 9.0, when compared with currents recorded at pHEXT 7.4. Error bars are SD. (C) Normalization to the module of the maximum current at each potentials revealed that at pHEXT 9.0 (blue diamonds), the current reversed at potential around −90 mV, as expected if protons are the charge carriers.

Mutant R223H. (A) Current recorded from Xenopus oocytes expressing the mutant R217Q-R223H-C363S. The currents are likely carried by protons as their amplitude increases with the acidification of the external medium. HP was 40 mV, and test pulses ranged from −140 to 50 mV. (B) Current amplitudes at the end of the test pulse were normalized with respect to the end-of-the-step amplitude recorded at −100 mV and pHEXT 7.4. Current displays several-fold increase at pHEXT 6.0, while decreasing at pHEXT 9.0, when compared with currents recorded at pHEXT 7.4. Error bars are SD. (C) Normalization to the module of the maximum current at each potentials revealed that at pHEXT 9.0 (blue diamonds), the current reversed at potential around −90 mV, as expected if protons are the charge carriers.

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