PDE-2 is required for ThRC adaptation. (A) ThRCs recorded in pde-2 mutant AFDs from a holding temperature of 17°C (top), 20°C (middle), or 22°C (bottom). V_hold = −60 mV. Arrows indicate T*. (B) Threshold for ThRC activation, T*, versus T_hold in wild-type (wt; black) and pde-2 (red) mutant AFD neurons. Lines are a linear fit to the data, showing a stronger correlation between T* and T_hold for wild type (R² = 0.69) compared with pde-2 (R² = 0.15). All animals were cultivated at 20°C. (C) Half-maximal duration plot versus T_hold from AFD neurons in wild-type and pde-2 mutants. R² = 0.31 for the linear fit from wild-type and R² = 0.19 from pde-2 mutants. (D) Peak amplitude of ThRCs versus T_hold for AFD neurons in wild-type and pde-2 mutants. R² = 0.006 for the linear fit from wild-type and R² = 0.04 from pde-2 mutants.