Figure 2. DIV voltage-sensor movement is... | Rockefeller University Press

Figure 2.

$Figure 2. DIV voltage-sensor movement is sufficient for fast inactivation. (A) A representative family of current traces in response to a 30-ms test pulse to −30 mV to assay the fraction of non–steady-state inactivated channels after a 100-ms conditioning prepulse between −170 and 50 mV. See Results for a detailed description of the voltage-pulse protocol. (B) Normalized steady-state inactivation versus conditioning prepulse voltage for wild-type and mutant channels. Solid lines are single Boltzmann fits to the mean for each construct (wild type: V1/2 = −62.3 mV and z = 4.7 e−; DI-CN: V1/2 = −69.2 mV and z = 4.2 e−; DII-CN: V1/2 = −65.8 mV and z = 3.7 e−; DIII-CN: V1/2 = −68.7 mV and z = 2.7 e−; DIV-CN: V1/2 = −137.3 mV and z = 1.1 e−). Note that the relation does not saturate above −200 mV for DIV-CN, so the observed left-shift in the steady-state inactivation versus voltage relation reflects the minimum shift conferred by this mutant.$

DIV voltage-sensor movement is sufficient for fast inactivation. (A) A representative family of current traces in response to a 30-ms test pulse to −30 mV to assay the fraction of non–steady-state inactivated channels after a 100-ms conditioning prepulse between −170 and 50 mV. See Results for a detailed description of the voltage-pulse protocol. (B) Normalized steady-state inactivation versus conditioning prepulse voltage for wild-type and mutant channels. Solid lines are single Boltzmann fits to the mean for each construct (wild type: V_1/2 = −62.3 mV and z = 4.7 e⁻; DI-CN: V_1/2 = −69.2 mV and z = 4.2 e⁻; DII-CN: V_1/2 = −65.8 mV and z = 3.7 e⁻; DIII-CN: V_1/2 = −68.7 mV and z = 2.7 e⁻; DIV-CN: V_1/2 = −137.3 mV and z = 1.1 e⁻). Note that the relation does not saturate above −200 mV for DIV-CN, so the observed left-shift in the steady-state inactivation versus voltage relation reflects the minimum shift conferred by this mutant.