UNC-8(H114Y), the trans-suppressor of UNC-(G387E) neuronal swelling, suppresses UNC-8(G387E) currents. (A) Example of currents recorded from an oocyte injected with UNC-8(G387E). (B) Example of currents recorded from an oocyte injected with UNC-8(G387E) plus UNC-8(H11Y). Both oocytes were perfused with the divalent cation–free plus EGTA NaCl solution. Currents were elicited by voltage steps from −160 to +100 mV in 20-mV increments from a holding potential of −30 mV. The dashed line corresponds to the zero current level. (C) Average current amplitudes at −160 mV recorded from oocytes injected with UNC-8(G387E) (n = 23), noninjected oocytes (n = 14), wild-type UNC-8 (n = 17), UNC-8(H114Y) (n = 23), UNC-8(G387E) plus UNC-8(wt) (n = 11), UNC-8(G387E) plus UNC-8(H114Y) (n = 23), and UNC-8(G387E/H114Y) (n = 13). **, P ≤ 0.01 by ANOVA. (D) Mean change in resting potential upon switch from NaCl physiological solution containing 1 mM CaCl₂ and 2 mM MgCl₂ to the divalent cation–free plus EGTA NaCl solution in oocytes injected with UNC-8(H114Y) (open diamonds; n = 5) and in oocytes injected with UNC-8(G387E) plus UNC-8(H114Y) (closed diamonds; n = 13).