Analysis of populations of PMNs in cancer patients by CyTOF. (A) Representative viSNE analysis of PMNs from tumors. CD66b⁺CD15⁺ PMNs were gated and the analysis of markers was performed within total population of PMNs. (B) Heatmap generated using raw median values of selected markers and proportion of PMN1 and PMN2 among all PMNs (n = 4). (C) The proportion of PMN1 and PMN2 populations among PMNs from tumors (n = 4). (D) Representative viSNE analysis of PMNs from peripheral blood of cancer patients. Analysis was performed as described in Fig. 9 A. (E) Heatmap generated using raw median values of selected markers and proportion of PMN1 and PMN2 among all PMNs (n = 4). (F) The proportion of PMN1 and PMN2 populations among PMNs from peripheral blood of cancer patients (n = 4). (G) The proportion of PMN1 and PMN2 populations among PMNs from peripheral blood of healthy donors (n = 4).