Morphology and phenotype of the populations of PMNs and CD14 expression and the function of the populations of PMNs in an acute infection model. (A) Giemsa staining of indicated populations of PMNs in LLC TB mice. Scale bars = 10 µm. (B) Expression of indicated molecules by flow cytometry in the populations of from tumors of LLC TB mice; 6 mice per group were analyzed. Frequency of CD14⁻, CD14^int, and CD14^high PMN differentiated from MLPG (n = 4). Mean and SD are shown. P values were calculated in unpaired two-sided Student’s t test. (D) Frequency of the populations of PMNs in spleens of mice 7 d after infection with Armstrong strain of LCMV; n = 5 mice per group. (E) Expression of arg1, nos2, ptgs2, and ptges by qPCR in control spleen CD14⁻ PMNs, as well as CD14⁻ and CD14^int PMNs isolated from spleen of LCMV-infected mice; n = 5 mice per group. (F) T cell proliferation in the presence of PMNs isolated from spleens of LCMV-infected mice (n = 3 mice per group). Mean and SEM are shown. *, P < 0.05; ****, P < 0.0001.