PMN clusters in TB mice. (A) Example of gating of PMNs. (B) Unsupervised clustering of PMNs. (C) Distribution of three PMN populations in spleen and tumors. (D) Expression of genes associated with immune suppression in different clusters of PMNs. (E) Expression of genes associated with cell activation and inflammation in different PMN clusters. Experiments were performed three times with spleen PMNs, three times with spleen PMNs from TB mice, and four times with tumor PMNs.