Double IF analysis of patient-derived melanocytic lesions. (A–D) IF staining of benign nevi (A, patients 1 and 2), dysplastic nevi (B, patients 3 and 4), primary melanoma (C, patient 6), and metastatic melanoma (D, patients 7, 8, and 9) skin tissues with anti-MelanA (green) and anti-AR (ab74272; red) antibodies, and topographically distinct areas (boxes 1, 2, and 3) used for single-cell AR expression quantification in Fig. 1 B. Shown are representative low- and high-magnification images of the areas used for quantification. Scale bar: 2 mm and 20 µm, respectively.