![Figure 14. Linkage analysis of modified Scheme 4 with modal-sensitive allosteric factors. (A) 〈q〉-V plots for Scheme 4 modified by ΔqG = 0.2 eo ([Ca2+]i = 10−3 to 103 µM; step size = 1.74×). The area of the dashed rectangle equals −4(ΔWC + ΔWE). (B) Corresponding energy Hill plot for J2 marker. Diverging V asymptotes are given by WH[j2]V(−) = −ΔGJ2 + kTln(ZCA/ZCR) + ΔqJ2V and WH[j2]V(+) = −(ΔGJ2 + ΔWG) + kTln(ZOA/ZOR) + (ΔqJ2 + ΔqG)V. These are further split by the lever operator μΔ into narrowly spaced parallel asymptotes whose height difference is −ΔWE. (C) 〈q〉-V plots for Scheme 4 modified by ΔqE = 0.2 eo ([Ca2+]i = 10−3 to 103 µM; step size = 1.74×). The difference in the areas of the two dashed rectangles is A2 − A1 = −4(ΔWC + ΔWE). (D) Corresponding energy Hill plot for J2 marker. The V asymptotes again diverge, given by WH[j2]V(−) = −ΔGJ2 + kTln((1 + K2)/ZCR) + ΔqJ2V and WH[j2]V(+) = −(ΔGJ2 + ΔGK1 + ΔWD + ΔWE + ΔWG) + kTln((1 + K2CF)/ZOR) + (ΔqJ2 + ΔqG)V + ΔnK1μ.](https://cdn.rupress.org/rup/content_public/journal/jgp/141/1/10.1085_jgp.201210859/4/jgp_201210859_fig14.jpeg?Expires=1767810673&Signature=PVh85zKkGRuaPv93VbqqwNDQIHClvfgEbK6RI0s~yc0QIxRuOl3i69wB0wILLaQdSEkLSCoUBq91Ty7jgqLzkW8lWYbW7ARwt37~ZuPAIp6yMarbaLpZ4xsnFNIfo9ordZ1x6UdN7ih8Zg6G~8rVKVVYCdxIIeWjuNhB3PEYE6HBFPXq9sfBwHfxBTUh8S5ZjSL5wjTnjQZWVOJXAX0EI2txQfghsUsohIHRQ7Qq-8h9FuKCGr1VObCweXO~X8TLf8dCz0kCxEwSZkuddv7DQrjhDUoxp3pXebZufJXTcEy6Q3omtx9bamsE4WJryv6EWKfvv7tswbhgxiuc1Prt8w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Linkage analysis of modified Scheme 4 with modal-sensitive allosteric factors. (A) 〈q〉-V plots for Scheme 4 modified by ΔqG = 0.2 eo ([Ca2+]i = 10−3 to 103 µM; step size = 1.74×). The area of the dashed rectangle equals −4(ΔWC + ΔWE). (B) Corresponding energy Hill plot for J2 marker. Diverging V asymptotes are given by WH[j2]V(−) = −ΔGJ2 + kTln(ZCA/ZCR) + ΔqJ2V and WH[j2]V(+) = −(ΔGJ2 + ΔWG) + kTln(ZOA/ZOR) + (ΔqJ2 + ΔqG)V. These are further split by the lever operator μΔ into narrowly spaced parallel asymptotes whose height difference is −ΔWE. (C) 〈q〉-V plots for Scheme 4 modified by ΔqE = 0.2 eo ([Ca2+]i = 10−3 to 103 µM; step size = 1.74×). The difference in the areas of the two dashed rectangles is A2 − A1 = −4(ΔWC + ΔWE). (D) Corresponding energy Hill plot for J2 marker. The V asymptotes again diverge, given by WH[j2]V(−) = −ΔGJ2 + kTln((1 + K2)/ZCR) + ΔqJ2V and WH[j2]V(+) = −(ΔGJ2 + ΔGK1 + ΔWD + ΔWE + ΔWG) + kTln((1 + K2CF)/ZOR) + (ΔqJ2 + ΔqG)V + ΔnK1μ.
