Figure 4. Representative experiments showing twitch tension (lowermost trace) and the furaptra fluorescence signal (upper traces) elicited by an AP in a mouse fast-twitch fiber (A) and a frog twitch fiber (B). Zero time marks the moment of the external shock; the tension trace in B has a small stimulus artifact. Peak amplitude, time of peak, and FDHM of ΔfCaD were 0.150, 5 ms, and 5 ms (A); and 0.164, 5 ms, and 12 ms (B). Fiber diameter, sarcomere spacing, and the number of sweeps averaged for each trace were 33 µm, 3.9 µm, and 2(A); and 54 µm, 3.8 µm, and 2, respectively (B). The labels and calibrations apply to both parts of the figure; the value 1.0 on the tension calibration corresponds to the peak of twitch tension.
Figure 4.

Representative experiments showing twitch tension (lowermost trace) and the furaptra fluorescence signal (upper traces) elicited by an AP in a mouse fast-twitch fiber (A) and a frog twitch fiber (B). Zero time marks the moment of the external shock; the tension trace in B has a small stimulus artifact. Peak amplitude, time of peak, and FDHM of ΔfCaD were 0.150, 5 ms, and 5 ms (A); and 0.164, 5 ms, and 12 ms (B). Fiber diameter, sarcomere spacing, and the number of sweeps averaged for each trace were 33 µm, 3.9 µm, and 2(A); and 54 µm, 3.8 µm, and 2, respectively (B). The labels and calibrations apply to both parts of the figure; the value 1.0 on the tension calibration corresponds to the peak of twitch tension.

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