Inactivation of α–β2 and α–mβ3a cross-linked complexes. (A–D) Representative macroscopic currents conducted by pWTα plus pWTβ2 (A) and cross-linked complexes between α S1 and β2 TM1 (B), α S2 and β2 TM1 (C), and α S0 and β2 TM2 (D). Measurements were made with inside-out macropatches using 10 µM Ca²⁺ inside the pipette. Currents were elicited by stepping the membrane voltage from a holding potential of −80 mV to depolarized test potentials in +20-mV increments up to +140 mV. (E) Summary graph of τ_inactivation at +160 mV. Mean ± SEM; n > 4; P = not significant by one-way ANOVA. (F–I) Representative macroscopic currents conducted by pWTα plus pWT mβ3a (F) and cross-linked complexes between α S1 and mβ3a TM1 (G), α S2 and mβ3a TM1 (H), and α S0 and mβ3a TM2 (I). Measurements were obtained as described above. (J) Summary graph of τ_inactivation at +160 mV. Mean ± SEM; n > 4; P = not significant by one-way ANOVA.