Figure 2.

Subcellular localization experiments. mTMEM16F-GFP expressed in HEK293 cells. The images were taken using an epifluorescence microscope. mTMEM16F-GFP was visualized with anti-GFP and Alexa Fluor 488. Anti-Hsp47 and Alexa Fluor 568 were used to label the ER, whereas the plasma membrane was stained using rhodamine-conjugated WGA. The nucleus was stained with DAPI.

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