Figure 8.
![Figure 8. Abrogation of inhibition of InsP3R-3 channel activity by physiological levels of [Ca2+]ER. (A) A typical single-channel current trace recorded during a switch of [Ca2+]ER from 70 nM to 1.1 mM. (B–D) Bar graphs of mean ratios of channel Po, to, and tc observed before and after [Ca2+]ER switches between 70 nM and 1.1 mM, showing the abrogation of inhibitory effects of 1.1 mM [Ca2+]ER on InsP3R channel activity in the presence of stronger cytoplasmic Ca2+ buffering (5 mM BAPTA) and smaller Ca2+ flux through the channel as a result of positive Vapp. Labels on the x axes indicate [diBrBAPTA] in mM (top) and Vapp in mV (bottom).](https://cdn.rupress.org/rup/content_public/journal/jgp/140/6/10.1085_jgp.201210804/5/jgp_201210804_fig8.jpeg?Expires=1767954853&Signature=xPdHe-OOv-0LjNqh~y6nJc1bHaN1MAVCHDuKCaxkcWlCRwQ7Qo~pXV-G2PPWZu5jECkBKitIDsfqxbWD-TxwI3yNdSYw2ARcA6YVvgyWDSkbCNBwx2GSrg1YD6rXqpeobKHGValtpGwSShbziNfQ5mLcUY7RCxhNxL4d7Ev5Rrhi8dnzd~pt1BcszVqBUOH7Cccnna~Hr29VfNALWDvbDjU0QHx4PRDajfa8Sgqu2FDcBI3t3VwbloMWwgJk4JgJjIWAUpzMw0aoidUQz~7CSk~7PJYsI--Xss931rnCPyJwzUWPgno-grLe0itkDazewRD0QREA4Pzay2dvAxn28w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Abrogation of inhibition of InsP3R-3 channel activity by physiological levels of [Ca2+]ER. (A) A typical single-channel current trace recorded during a switch of [Ca2+]ER from 70 nM to 1.1 mM. (B–D) Bar graphs of mean ratios of channel Po, to, and tc observed before and after [Ca2+]ER switches between 70 nM and 1.1 mM, showing the abrogation of inhibitory effects of 1.1 mM [Ca2+]ER on InsP3R channel activity in the presence of stronger cytoplasmic Ca2+ buffering (5 mM BAPTA) and smaller Ca2+ flux through the channel as a result of positive Vapp. Labels on the x axes indicate [diBrBAPTA] in mM (top) and Vapp in mV (bottom).
