Figure 6.
![Figure 6. Effects of cytoplasmic Ca2+-buffering conditions on [Ca2+]ER modulation of InsP3R channel activity. (A and B) Typical single-channel lum-out nuclear patch-clamp current traces recorded during switches of [Ca2+]ER from 70 nM to 300 µM in different cytoplasmic Ca2+-buffering conditions. (C–E) Bar graphs of mean ratios of Po, to, and tc, respectively, observed before and after [Ca2+]ER switches between 70 nM and 300 µM for different cytoplasmic Ca2+-buffering conditions. oo indicates statistically significant difference between the two ratios connected by the bracket (P < 0.01; unpaired t test). Note the logarithmic scale used for the tc axis (in red) in E.](https://cdn.rupress.org/rup/content_public/journal/jgp/140/6/10.1085_jgp.201210804/5/jgp_201210804_fig6.jpeg?Expires=1767940877&Signature=hCPnphODT6~~oHItGCUBDIKiIwTPVntJnI1pclVGi-oftofLqbMuhQILEGjyvvOs8M60rK91--EB8fNDuXMVFCr2Iu7W7uC6uvKQnAkexx-WobptCbzQICHPLE2kUXSQAOpAw4g1rKJykNepB2BUIj42emyNWxD44WYL~ErURW7KvKGXVt9U-tQ-6U9h6zR-uOUYcoV-DFNIP~v~qqqlkeCWz9GPxrGZaMDnN5fctNXL1z4C9EaerkzR7abRGZkmGxor88DlX1sNCpafMnP2v6uu~gFf47Rzw~PfuTKmMsqNsoEl04pGfv0H4BvVV-Y39hTSocjNktAm0MMA6OIvxw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of cytoplasmic Ca2+-buffering conditions on [Ca2+]ER modulation of InsP3R channel activity. (A and B) Typical single-channel lum-out nuclear patch-clamp current traces recorded during switches of [Ca2+]ER from 70 nM to 300 µM in different cytoplasmic Ca2+-buffering conditions. (C–E) Bar graphs of mean ratios of Po, to, and tc, respectively, observed before and after [Ca2+]ER switches between 70 nM and 300 µM for different cytoplasmic Ca2+-buffering conditions. oo indicates statistically significant difference between the two ratios connected by the bracket (P < 0.01; unpaired t test). Note the logarithmic scale used for the tc axis (in red) in E.
