Figure 4.
![Figure 4. Calcium dependence of mitochondrial Ca2+ uptake in the absence (A) and presence (B) of 10 nM Ru360. [Ca2+]out and [Ca2+]mito were monitored during mitochondrial Ca2+ uptake initiated by Ca2+ additions ranging from 0.2 (black), to 0.55 (red), to 2 (blue), to 5.7 µM (green). 10 nM Ru360 largely eliminated MCUmode2 (see [Ca2+]out in bottom panels) but only partially inhibited MCUmode1 ([Ca2+]mito response in top panels). Standard KCl-based buffer with 2 mM KH2PO4, 5 mM G/M, 5 mM NaCl, 10 µM CGP, 1 µM CsA, and 2.5 µM thapsigargin.](https://cdn.rupress.org/rup/content_public/journal/jgp/139/6/10.1085_jgp.201210784/4/jgp_201210784_fig4.jpeg?Expires=1767753972&Signature=h18HA1lYZ4wlL0uFwlmK1JFDeVUIlSaZvbV~Yx1QbebigMzzOfkd6joq3euRaJ4~95RAI1noMA0-W1Q7H5vf3J4uky-bDrIRGSvOs8jWo~7BUkb-AOXeEs5dA0pkbBEghyluYhKrPPvVhcFME5VpIA~Hp79cwRrifxsbO~uk2NdQ4mfQ~qkTcryJivOthKdsQz4wuCmmcdDUt8yR0Ke2nzRoP2xMeTrXvBijo1OA1J~g25kR50MM~qdKBo9CFWgby~w-f7-wvVhMG~VhKc4CTUgegmwnRJloGNrCwJsbfJQeGI-bS2fwJ~ofWzA2Cc992~jkHnmO8e8ZcT3bxGVm~g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Calcium dependence of mitochondrial Ca2+ uptake in the absence (A) and presence (B) of 10 nM Ru360. [Ca2+]out and [Ca2+]mito were monitored during mitochondrial Ca2+ uptake initiated by Ca2+ additions ranging from 0.2 (black), to 0.55 (red), to 2 (blue), to 5.7 µM (green). 10 nM Ru360 largely eliminated MCUmode2 (see [Ca2+]out in bottom panels) but only partially inhibited MCUmode1 ([Ca2+]mito response in top panels). Standard KCl-based buffer with 2 mM KH2PO4, 5 mM G/M, 5 mM NaCl, 10 µM CGP, 1 µM CsA, and 2.5 µM thapsigargin.
