Figure 3.
![Figure 3. Concentration dependence of Ru360 inhibition of mitochondrial Ca2+ uptake. Fura-FF–loaded mitochondria were preincubated with 0 (black), 2 (red), 10 (blue), 100 (magenta), or 1,000 nM (green) Ru360 in the standard KCl-based buffer with 2 mM KH2PO4, 5 mM G/M, 5 mM NaCl, 10 µM CGP37157, 1 µM CsA, and 2.5 µM thapsigargin. 2 µM free Ca2+ was added to initiate mitochondrial Ca2+ uptake and [Ca2+]out (A) and [Ca2+]mito (B) were monitored simultaneously.](https://cdn.rupress.org/rup/content_public/journal/jgp/139/6/10.1085_jgp.201210784/4/jgp_201210784_fig3.jpeg?Expires=1767751719&Signature=b4Pp3Y41Xb7tPTTPaKVS8id5nLytCXTOiTdaUXDbQogZvuTPyyPJMu-Iy0XxwpkUX9Ah2mr8V5YV6i4LnI2LEssDUx6j-vebGTf0W5TQCKKBwMCWCcekRJ6FUWH5xh-mcZRgARvwETGArBOCL~nWoL~4WxAjSxREk6i0eo46tZekKyXjSLNRu1C~L767cy7Lqhw4iEyiqyGZf0MbGQQnhafVNM~a-dbiz6vb5Wec7K0AEqH7DHtXpfEpu1w1LFQh7I1ziFYZFtY~tdrhtd9ZACCEyRSo3-8SG07PawQOZp~-6xFp9LPoGyQlZdq8c7XreAlAvaCVdEdleZIo~-iTcA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Concentration dependence of Ru360 inhibition of mitochondrial Ca2+ uptake. Fura-FF–loaded mitochondria were preincubated with 0 (black), 2 (red), 10 (blue), 100 (magenta), or 1,000 nM (green) Ru360 in the standard KCl-based buffer with 2 mM KH2PO4, 5 mM G/M, 5 mM NaCl, 10 µM CGP37157, 1 µM CsA, and 2.5 µM thapsigargin. 2 µM free Ca2+ was added to initiate mitochondrial Ca2+ uptake and [Ca2+]out (A) and [Ca2+]mito (B) were monitored simultaneously.
