Homology model of NaChBac. (A) Sequence alignment of the pore region (S5–selectivity filter–S6 segments) of NaChBac aligned with NavAb. SF, selectivity filter. Stars indicate conservation, double dots show strong conservation, and single dots indicate weak conservation. (B) A homology model of the pore region of NaChBac generated based on the crystal structure of NavAb. The structure is shown with a top view (left) and a side view (right), with one monomer removed for clarity.