Voltage dependence of the rate of return of stabilized charge. (A) Representative current traces from a paired-pulse protocol with extending durations between test pulses reveals the slow recovery of IgON at −100 mV. A 12-ms depolarizing pulse to 30 mV was given to move all the charge and induce charge stabilization. This was followed by an extending recovery period, after which a test pulse to 30 mV was reapplied to assess the amount of charge that had returned. (B) The recovery of charge was plotted against the recovery pulse intervals at different holding potentials (HP) and fit with a single exponential. (C) The mean IgOFF rates were plotted against the recovery potential and fit with an exponential function of voltage (Eq. 1). The time constants of the fitted exponential functions were, at −100 mV, τ = 17.2 ± 1.7 ms (n = 5); at −90 mV, τ = 31 ± 0.6 ms (n = 3); and at −80 mV, τ = 62.4 ± 0.6 ms (n = 5). Error bars represent SEM.
