Time-dependent and steady-state behavior of the computational model of GSH/Trx systems. (A) The scheme of the computational model as described in Materials and methods. (B) The continuation analysis of the steady-state behavior as a function of the rate of H₂O₂ provision by SOD activity (V_SOD); the latter was varied between 5 × 10⁻⁵ and 5.5 × 10⁻⁴ mM ms⁻¹, with $kTrxR1$ = 0.022 ms⁻¹ and $kGR1$ = 0.025 ms⁻¹. (C) Time-dependent simulations showing the increase in the emission of H₂O₂ upon inhibition of $kTrxR1$ and $kGR1$⁠, which were adjusted from 0.022 to 0.009 ms⁻¹ and 0.025 to 0.005 ms⁻¹, respectively, to represent the inhibitory action of AF and DNCB, respectively.