Calculation of IKV-dependent attenuation of fluorescence transients. The attenuations in di-8-ANEPPS transients are exemplified with two pulse amplitudes (100 mV in A and 200 mV in B). (A) The red and black traces are the di-8-ANEPPS transients recorded in the presence of NMG-Tyrode and TEA-Tyrode, respectively. The green trace represents the attenuation (percentage), calculated by subtracting the red from the black traces and dividing the result by the amplitude of the black trace in B. (B) Same as in A, but for a depolarization to 110 mV. The inset shows the initial part of the records in an expended timescale. (C and D) The currents recorded simultaneously with the optical data in A and B are shown (with the same color code) in C and D, respectively. To reduce the increment of noise inherent to the subtraction operation, the attenuation traces (green traces) were digitally filtered.
