Figure 7.
Figure 7. Effects of IKV activation on di-8-ANEPPS transients. (A) di-8-ANEPPS transients recorded in response to 500-ms depolarizations from VH to membrane potentials spanning −90–70 mV in 40-mV steps. The inset shows the early phases of fluorescence transients in an expanded timescale. (B) Fluorescence transients elicited in the same fiber as in A, and using the same pulse protocol, after exchanging NMG-Tyrode with TEA-Tyrode. (C and D) The currents recorded simultaneously with the optical records in A and B are shown in C and D, respectively. For both fluorescence and current records, green, black, red, blue, and orange traces correspond to responses to depolarization to −90, −50, −10, 30, and 70 mV, respectively.

Effects of IKV activation on di-8-ANEPPS transients. (A) di-8-ANEPPS transients recorded in response to 500-ms depolarizations from VH to membrane potentials spanning −90–70 mV in 40-mV steps. The inset shows the early phases of fluorescence transients in an expanded timescale. (B) Fluorescence transients elicited in the same fiber as in A, and using the same pulse protocol, after exchanging NMG-Tyrode with TEA-Tyrode. (C and D) The currents recorded simultaneously with the optical records in A and B are shown in C and D, respectively. For both fluorescence and current records, green, black, red, blue, and orange traces correspond to responses to depolarization to −90, −50, −10, 30, and 70 mV, respectively.

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