Modulation of KV1 family channels by depletion of PI(4,5)P2 at the plasma membrane. (A) Potassium outward currents in KV1.1/KVβ1.1 channel complexes coexpressed with M1R. Black indicates control current trace in Ringer’s solution, red indicates during application of 10 µM Oxo-M, and green indicates after washout of Oxo-M. Arrows indicate points at which peak and steady-state current amplitudes were measured. (B) Current amplitudes of peak and steady-state current of the cell shown in A over time. (C) Percent increase of peak and steady-state currents of KV1.1/KVβ1.1 channel complexes coexpressed with M1R upon stimulation with Oxo-M (n = 7). Error bars represent ±SEM. *, P < 0.05. (D) Current time course in a cell expressing KV1.1/KVβ1.1 channel complexes and Dr-VSP. Dr-VSP was activated by a 2-s pulse to 100 mV. Test pulse protocol as shown in A. (E) Same as D but with expression of pseudojanin-YFP and LDR-CFP instead of Dr-VSP and addition of 5 µM rapamycin.
